Afacultative anaerobic organism is an organism that makes ATPbyaerobic respirationifoxygen is present, but is capable of switching to fermentation if oxygen is absent.[1][2]
Some examples of facultatively anaerobic bacteria are Staphylococcus spp.,[3] Escherichia coli, Salmonella, Listeria spp.,[4] Shewanella oneidensis and Yersinia pestis. Certain eukaryotes are also facultative anaerobes, including fungi such as Saccharomyces cerevisiae[5] and many aquatic invertebrates such as nereid polychaetes.[6]
It has been observed that in mutants of Salmonella typhimurium that underwent mutations to be either obligate aerobes or anaerobes, there were varying levels of chromatin-remodeling proteins. The obligate aerobes were later found to have a defective DNA gyrase subunit A gene (gyrA), while obligate anaerobes were defective in topoisomerase I (topI). This indicates that topoisomerase I and its associated relaxation of chromosomal DNA is required for transcription of genes required for aerobic growth, while the opposite is true for DNA gyrase.[7] Additionally, in Escherichia coli K-12 it has been noted that phosphofructokinase (PFK) exists as a dimer under aerobic conditions and as a tetramer under anaerobic conditions. Given PFK’s role in glycolysis, this has implications for the effect of oxygen on the glucose metabolism of E. coli K-12 in relation to the mechanism of the Pasteur effect.[8][9]
There may exist a core network of transcription factors (TFs) that includes the major oxygen-responsive ArcA and FNR control the adaptation of Escherichia coli to changes in oxygen availability. Activities of these two regulators are indicative of spatial effects that may affect gene expression in the microaerobic range. It has also been observed that these oxygen-sensitive proteins are protected within the cytoplasm by oxygen consumers within the cell membrane, known as terminal oxidases.[10]
Facultative anaerobes are able to grow in both the presence and absence of oxygen due to the expression of both aerobic and anaerobic respiratory chains using either oxygen or an alternative electron acceptor.[11] For example, in the absence of oxygen, E. coli can use fumarate, nitrate, nitrite, dimethyl sulfoxide, or trimethylamine oxide as an electron acceptor.[11] This flexibility allows facultative anaerobes to survive in a number of environments, and in environments with frequently changing conditions.[1]
Several species of protists use a facultative anaerobic metabolism to enhance their ATP production, and some can produce dihydrogen through this process.[12]
Since facultative anaerobes are able to grow in both the presence and absence of oxygen, they can survive in many different environments, adapt easily to changing conditions, and thus have a selective advantage over other bacteria. As a result, most life-threatening pathogens are facultative anaerobes.[1]
The ability of facultative anaerobic pathogens to survive without oxygen is important since their infection is shown to reduce oxygen levels in their host's gut tissue.[13] Moreover, the ability of facultative anaerobes to limit oxygen levels at infection sites is beneficial to them and other bacteria, as dioxygen can form reactive oxygen species (ROS). These species are toxic to bacteria and can damage their DNA, among other constituents.[1]
