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The '''Ligase chain reaction''' (LCR) is a method of [[DNA amplification]]. While the better known [[PCR]] carries out the amplificiation by polymerizing nucleotides, this method instead amplifies the nucleic acid used as the probe. For each of the two DNA strands, two partial probes are ligated to form the actual one; thus, this method uses two enzymes: a [[DNA polymerase]] and a [[DNA ligase]]. Each cycle results in a doubling of the target nucleic acid molecule. A key advantage of LCR is increase specificity as compared to PCR.<ref>Wiedmann M, Wilson WJ, Czajka J, Luo J, Barany F, Batt CA. "Ligase chain reaction (LCR)--overview and applications." '' PCR Methods and Applications'' 1994 Feb;3(4):S51-64 PMID: 8173509</ref>. It has been widely used for the detection of [[Point mutation|single base mutation]]s, as in [[genetic disease]]s. <ref>Barany F. "Genetic disease detection and DNA amplification using cloned thermostable ligase." ''Proc Natl Acad Sci U S A.'' 1991 Jan 1;88(1):189-93. PMID: 1986365 </ref> |
The '''Ligase chain reaction''' (LCR) is a method of [[DNA amplification]]. While the better known [[PCR]] carries out the amplificiation by polymerizing nucleotides, this method instead amplifies the nucleic acid used as the probe. For each of the two DNA strands, two partial probes are ligated to form the actual one; thus, this method uses two enzymes: a [[DNA polymerase]] and a [[DNA ligase]]. Each cycle results in a doubling of the target nucleic acid molecule. A key advantage of LCR is increase specificity as compared to PCR.<ref>Wiedmann M, Wilson WJ, Czajka J, Luo J, Barany F, Batt CA. "Ligase chain reaction (LCR)--overview and applications." '' PCR Methods and Applications'' 1994 Feb;3(4):S51-64 PMID: 8173509</ref>. It has been widely used for the detection of [[Point mutation|single base mutation]]s, as in [[genetic disease]]s. <ref>Barany F. "Genetic disease detection and DNA amplification using cloned thermostable ligase." ''Proc Natl Acad Sci U S A.'' 1991 Jan 1;88(1):189-93. PMID: 1986365 </ref> |
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==General reading== |
==General reading== |
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*Walker, J. M., & Rapley, R. (2005). Medical biomethods handbook. Totowa, N.J.: Humana Press. ISBN 9781592598700 |
*Walker, J. M., & Rapley, R. (2005). Medical biomethods handbook. Totowa, N.J.: Humana Press. ISBN 9781592598700 |
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[[Category:DNA profiling techniques]] |
[[Category:DNA profiling techniques]] |
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[[Category:Amplifiers]] |
[[Category:Amplifiers]] |
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The Ligase chain reaction (LCR) is a method of DNA amplification. While the better known PCR carries out the amplificiation by polymerizing nucleotides, this method instead amplifies the nucleic acid used as the probe. For each of the two DNA strands, two partial probes are ligated to form the actual one; thus, this method uses two enzymes: a DNA polymerase and a DNA ligase. Each cycle results in a doubling of the target nucleic acid molecule. A key advantage of LCR is increase specificity as compared to PCR.[1]. It has been widely used for the detection of single base mutations, as in genetic diseases. [2]
