Neutravidin is a commercial brand by thermofisher. The non commercial name for this derivative is Neutralite avidin
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== References == |
== References == |
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* Bayer, Ed: "[https://www.weizmann.ac.il/Biomolecular_Sciences/Bayer/research-activities/avidin-biotin-system The avidin-biotin system]", Dept. of Biological Chemistry, Weizmann Institute of Science, Israel |
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* {{Literatur|Autor=Y. Hiller, J.M. Gershoni, E. A. Bayer, M. Wilchek|Titel=Biotin binding to avidin. Oligosaccharide side chain not required for ligand association|Sammelwerk=[[Biochemical Journal]]|Band=248|Nummer=1|Datum=1987|DOI=10.1042/bj2480167|PMC=1148514|PMID=3435435|Seiten=167–171}} |
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* {{Literatur|Autor=Edward A. Bayer, Fabien De Meester, Tikva Kulik, Meir Wilchek|Titel=Preparation of deglycosylated egg white avidin|Sammelwerk=Applied Biochemistry and Biotechnology|Band=53|Nummer=1|Datum=1995|DOI=10.1007/BF02783477|Seiten=1–9}} |
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Y. Hiller, J.M. Gershoni, E.A. Bayer, M. Wilchek Biochem. J., 248 (1987), pp. 167–171 |
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* {{Literatur|Autor=Ari T. Marttila, Olli H. Laitinen, Kari J. Airenne, Tikva Kulik, Edward A. Bayer, Meir Wilchek, Markku S. Kulomaa|Titel=Recombinant NeutraLite Avidin: a non‐glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non‐specific binding properties|Sammelwerk=FEBS Letters|Band=467|Nummer=1|Datum=2000|DOI=10.1016/S0014-5793(00)01119-4|Seiten=31–36}} |
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E.A. Bayer, F. De Meester, T. Kulik, M. Wilchek Appl. Biochem. Biotechnol., 53 (1995), pp. 1–9 |
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A.T. Marttilaa et al. FEBS Letters 467 (2000), pp. 31–36 Recombinant NeutraLite Avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties |
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[[Category:Proteins]] |
[[Category:Proteins]] |
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Neutralite Avidin protein is a deglycosylated version of chicken avidin, with a mass of approximately 60,000 daltons. As a result of carbohydrate removal, lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is not necessary for this activity. Avidin has a high pI but NeutrAvidin has a near-neutral pI (pH 6.3), minimizing non-specific interactions with the negatively-charged cell surface or with DNA/RNA. Neutravidin still has lysine residues that remain available for derivatizationorconjugation.
Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin (Kd = 10−15 M). In biochemical applications, streptavidin, which also binds very tightly to biotin, may be used interchangeably with NeutrAvidin.
Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane-impermeable biotin reagent, then specifically captured using a NeutrAvidin support.
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