Contents

Proteasome (prosome, macropain) subunit, alpha 1

Protein-coding gene in the species Homo sapiens

PSMA1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4R3O, 4R67, 1IRU, 5A0Q
Identifiers
Aliases	PSMA1, HC2, HEL-S-275, NU, PROS30, Proteasome (prosome, macropain) subunit, alpha 1, proteasome subunit alpha 1, proteasome 20S subunit alpha 1
External IDs	OMIM: 602854; MGI: 1347005; HomoloGene: 2080; GeneCards: PSMA1; OMA:PSMA1 - orthologs
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.2 Start 14,504,874 bp[1] End 14,643,635 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 F1|7 59.32 cM Start 113,863,841 bp[2] End 113,875,353 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in islet of Langerhans smooth muscle tissue lymph node rectum appendix endometrium gastrocnemius muscle Descending thoracic aorta right adrenal cortex monocyte Top expressed in otic placode abdominal wall medial ganglionic eminence endocardial cushion mandibular prominence maxillary prominence precursor cell fetal liver hematopoietic progenitor cell migratory enteric neural crest cell efferent ductule More reference expression data BioGPS More reference expression data
Gene ontology Molecular function endopeptidase activity peptidase activity protein binding RNA binding threonine-type endopeptidase activity hydrolase activity lipopolysaccharide binding Cellular component cytoplasm polysome cytosol centrosome nucleoplasm proteasome complex extracellular exosome nucleus proteasome core complex proteasome core complex, alpha-subunit complex Biological process regulation of cellular amino acid metabolic process antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent ubiquitin-dependent protein catabolic process regulation of mRNA stability immune system process positive regulation of canonical Wnt signaling pathway protein polyubiquitination stimulatory C-type lectin receptor signaling pathway tumor necrosis factor-mediated signaling pathway proteolysis MAPK cascade Fc-epsilon receptor signaling pathway NIK/NF-kappaB signaling anaphase-promoting complex-dependent catabolic process proteolysis involved in cellular protein catabolic process T cell receptor signaling pathway negative regulation of canonical Wnt signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process Wnt signaling pathway, planar cell polarity pathway negative regulation of G2/M transition of mitotic cell cycle protein deubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process transmembrane transport regulation of transcription from RNA polymerase II promoter in response to hypoxia post-translational protein modification regulation of hematopoietic stem cell differentiation negative regulation of inflammatory response to antigenic stimulus proteasomal protein catabolic process proteasomal ubiquitin-independent protein catabolic process interleukin-1-mediated signaling pathway regulation of mitotic cell cycle phase transition Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5682 26440 Ensembl ENSG00000129084 ENSMUSG00000030751 UniProt P25786 Q9R1P4 RefSeq (mRNA) NM_148976 NM_001143937 NM_002786 NM_011965 RefSeq (protein) NP_001137409 NP_002777 NP_683877 NP_036095 Location (UCSC) Chr 11: 14.5 – 14.64 Mb Chr 7: 113.86 – 113.88 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Proteasome subunit alpha type-1 is a protein that in humans is encoded by the PSMA1 gene.^[5][6][7] This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, and beta5i) that contributes to the complete assembly of 20S proteasome complex.

Structure[edit]

Protein expression[edit]

Th gene PSMA1 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.^[7] In a study of mouse gene PSMA1 which share 98% homology with human gene, the gene was isolated and cloned, and then identified as C2 subunit of the 20S proteasome (old nomenclature). The gene has 10 exons that are distributed over a 12kb region on mouse chromosome 7. The same study showed that mouse genes Psma1 and Pde3b are closely linked, residing between cM 53 and 53.3 in a region syntenic to human chromosome 11p15. The human protein proteasome subunit alpha type-1 is also known as 20S proteasome subunit alpha-6 (based on systematic nomenclature). The protein is 30 kDa in size and composed of 263 amino acids. The calculated theoretical pI of this protein is 6.15.

Complex assembly[edit]

The proteasome is a multicatalytic proteinase complex with a highly ordered 20S core structure. This barrel-shaped core structure is composed of 4 axially stacked rings of 28 non-identical subunits: The two end rings are each formed by 7 alpha subunits, and the two central rings are each formed by 7 beta subunits. Three beta subunits (beta1, beta2, and beta5) each contains a proteolytic active site. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.^[8][9]

Function[edit]

Crystal structures of isolated 20S proteasome complex demonstrate that the two rings of beta subunits form a proteolytic chamber and maintain all their active sites of proteolysis within the chamber.^[9] Concomitantly, the rings of alpha subunits form the entrance of substrate entering the proteolytic chamber. In an inactivated 20S proteasome complex, the gate into the internal proteolytic chamber are guarded by N-terminal tails of specific alpha-subunit.^[10][11] The proteolytic capacity of 20S core particle (CP) can be activated when CP associates with one or two regulatory particles (RP) on one or both side of alpha rings. These regulatory particles include 19S proteasome complexes, 11S proteasome complex, etc. Following the CP-RP association, the confirmation of certain alpha subunits will change and consequently cause the opening of substrate entrance gate. Besides RPs, the 20S proteasomes can also be effectively activated by other mild chemical treatments, such as exposure to low levels of sodium dodecylsulfate (SDS).^[11] As a component of alpha ring, Proteasome subunit alpha type-1 contributes to the formation of heptameric alpha rings and substrate entrance gate. The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Clinical significance[edit]

The proteasome and its subunits are of clinical significance for at least two reasons: (1) a compromised complex assembly or a dysfunctional proteasome can be associated with the underlying pathophysiology of specific diseases, and (2) they can be exploited as drug targets for therapeutic interventions. More recently, effort has been made to consider the proteasome for the development of novel diagnostic markers and strategies. An improved and comprehensive understanding of the pathophysiology of the proteasome should lead to clinical applications in the future.

The proteasomes form a pivotal component for the ubiquitin–proteasome system (UPS) ^[12] and corresponding cellular Protein Quality Control (PQC). Protein ubiquitination and subsequent proteolysis and degradation by the proteasome are important mechanisms in the regulation of the cell cycle, cell growth and differentiation, gene transcription, signal transduction and apoptosis.^[13] Subsequently, a compromised proteasome complex assembly and function lead to reduced proteolytic activities and the accumulation of damaged or misfolded protein species. Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases,^[14][15] cardiovascular diseases,^[16][17][18] inflammatory responses and autoimmune diseases,^[19] and systemic DNA damage responses leading to malignancies.^[20]

Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the pathogenesis of several neurodegenerative and myodegenerative disorders, including Alzheimer's disease,^[21] Parkinson's disease^[22] and Pick's disease,^[23] Amyotrophic lateral sclerosis (ALS),^[23] Huntington's disease,^[22] Creutzfeldt–Jakob disease,^[24] and motor neuron diseases, polyglutamine (PolyQ) diseases, Muscular dystrophies^[25] and several rare forms of neurodegenerative diseases associated with dementia.^[26] As part of the ubiquitin–proteasome system (UPS), the proteasome maintains cardiac protein homeostasis and thus plays a significant role in cardiac ischemic injury,^[27] ventricular hypertrophy^[28] and heart failure.^[29] Additionally, evidence is accumulating that the UPS plays an essential role in malignant transformation. UPS proteolysis plays a major role in responses of cancer cells to stimulatory signals that are critical for the development of cancer. Accordingly, gene expression by degradation of transcription factors, such as p53, c-jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, STAT3, sterol-regulated element-binding proteins and androgen receptors are all controlled by the UPS and thus involved in the development of various malignancies.^[30] Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in colorectal cancer, retinoblastoma (Rb). and von Hippel–Lindau tumor suppressor (VHL), as well as a number of proto-oncogenes (Raf, Myc, Myb, Rel, Src, Mos, ABL). The UPS is also involved in the regulation of inflammatory responses. This activity is usually attributed to the role of proteasomes in the activation of NF-κB which further regulates the expression of pro inflammatory cytokines such as TNF-α, IL-β, IL-8, adhesion molecules (ICAM-1, VCAM-1, P-selectin) and prostaglandins and nitric oxide (NO).^[19] Additionally, the UPS also plays a role in inflammatory responses as regulators of leukocyte proliferation, mainly through proteolysis of cyclines and the degradation of CDK inhibitors.^[31] Lastly, autoimmune disease patients with SLE, Sjögren syndrome and rheumatoid arthritis (RA) predominantly exhibit circulating proteasomes which can be applied as clinical biomarkers.^[32]

Radiation therapy is a critical modality in the treatment of cancer. Accordingly, the proteasome subunit alpha type-1 was examined as a strategy in radio sensitizing for the treatment of non-small-cell lung carcinomas. Proteasome inhibition through the knockdown of PSMA1 resulted in loss of protein expression of the proteasome subunit alpha type-1 and the proteasome chymotrypsin-like activity. A combination of PSMA1 knockdown in parallel with radiation therapy to treat non-small cell lung carcinoma resulted in an increased sensitivity of the tumor to radiation and improved tumor control.^[33] The study suggests that proteasome inhibition through PSMA1 knockdown is a promising strategy for non-small cell lung carcinomas radiosensitization via inhibition of NF-κB-mediated expression of Fanconi anemia/HR DNA repair genes.^[33]

References[edit]

Further reading[edit]