The Warthin–Starry stain (WS) is a silver nitrate-based staining method (asilver stain) used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin (1866–1931) and Allen Chronister Starry (1890–1973), for the detection of spirochetes.[1][2] It has been considered a standard stain for the detection of spirochetes,[3] and is also used to stain Helicobacter pylori, Lawsonia intracellularis,[4] Microsporidia,[5][6] and particulates.[7] It is also important for confirmation of Bartonella henselae, a causative organism in cat-scratch disease.
Warthin–Starry stains organisms dark brown to black, and the background light golden brown/golden yellow.[5]
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| Iron/hemosiderin |
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| Lipids |
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| Amyloid |
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| Bacteria |
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| Connective tissue |
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| Other |
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| Tissue stainability |
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