This enzyme belongs to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. The systematic name of this enzyme class is O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase. Other names in common use include O-acetyl-L-serine sulfhydrylase, O-acetyl-L-serine sulfohydrolase, O-acetylserine (thiol)-lyase, O-acetylserine (thiol)-lyase A, O-acetylserine sulfhydrylase, O3-acetyl-L-serine acetate-lyase (adding hydrogen-sulfide), acetylserine sulfhydrylase, cysteine synthetase, S-sulfocysteine synthase, 3-O-acetyl-L-serine:hydrogen-sulfide, and 2-amino-2-carboxyethyltransferase. This enzyme participates in 3 metabolic pathways: cysteine metabolism, selenoamino acid metabolism, and sulfur metabolism. It employs one cofactor, pyridoxal phosphate.
Becker MA, Kredich NM, Tomkins GM (1969). "The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium". J. Biol. Chem. 244 (9): 2418–27. PMID4891157.
Hara S, Payne MA, Schnackerz KD, Cook PF (1990). "A rapid purification procedure and computer-assisted sulfide ion selective electrode assay for O-acetylserine sulfhydrylase from Salmonella typhimurium". Protein Expr. Purif. 1 (1): 70–6. doi:10.1016/1046-5928(90)90048-4. PMID2152186.
Ikegami F, Kaneko M, Lambein F, Kuo Y-H, Murakoshi I (1987). "Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum". Phytochemistry. 26 (10): 2699–2704. doi:10.1016/S0031-9422(00)83575-X.
Murakoshi I, Kaneko M, Koide C, Ikegami F (1986). "Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase". Phytochemistry. 25 (12): 2759–2763. doi:10.1016/S0031-9422(00)83736-X.
Tai CH, Burkhard P, Gani D, Jenn T, Johnson C, Cook PF (2001). "Characterization of the allosteric anion-binding site of O-acetylserine sulfhydrylase". Biochemistry. 40 (25): 7446–52. doi:10.1021/bi015511s. PMID11412097.
L-cysteine + acetate