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EIF1

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Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1.^[5]^[6]^[7]

EIF1

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
2IF1, 4KZX, 4KZY

Identifiers

EIF1, A121, EIF-1, ISO1, SUI1, eukaryotic translation initiation factor 1, Eukaryotic initiation factor 1

External IDs

MGI: 105125; HomoloGene: 130538; GeneCards: EIF1; OMA:EIF1 - orthologs

Gene location (Human)

Chr.	Chromosome 17 (human)^[1]

Band	17q21.2	Start	41,688,885 bp^[1]
Band	17q21.2	End	41,692,668 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 11 (mouse)^[2]

Band	11\|11 D	Start	100,210,711 bp^[2]
Band	11\|11 D	End	100,212,922 bp^[2]

RNA expression pattern

Human

Mouse (ortholog)

Top expressed in

sperm

oocyte

parotid gland

parietal pleura

tail of epididymis

lower lobe of lung

secondary oocyte

vena cava

caput epididymis

visceral pleura

Top expressed in

granulocyte

muscle of thigh

morula

blastocyst

superior frontal gyrus

right kidney

ventricular zone

neural tube

extensor digitorum longus muscle

embryo

More reference expression data

n/a

Gene ontology
Molecular function	translation factor activity, RNA binding translation initiation factor activity RNA binding protein binding ribosomal small subunit binding
Cellular component	cytoplasm nucleus eukaryotic 43S preinitiation complex
Biological process	translational initiation response to stress dosage compensation by inactivation of X chromosome protein biosynthesis regulation of translational initiation
Sources:Amigo / QuickGO

Species

Human

Mouse


10209


20918


ENSG00000173812


ENSMUSG00000035530


P41567


P48024

RefSeq (mRNA)


NM_005801


NM_011508

RefSeq (protein)


NP_005792


NP_035638

Location (UCSC)

Chr 17: 41.69 – 41.69 Mb

Chr 11: 100.21 – 100.21 Mb

PubMed search

^[3]

^[4]

View/Edit Human

View/Edit Mouse

eIF1 interacts with the eukaryotic small (40S) ribosomal subunit and eIF3, and is a component of the 43S preinitiation complex (PIC).^[8] eIF1 and eIF1A bind cooperatively to the 40S to stabilize an "open" conformation of the preinitiation complex (PIC) during eukaryotic translation initiation.^[8] eIF1 binds to a region near the ribosomal P-site in the 40S subunit and functions in a manner similar to the structurally related bacterial counterpart IF3.^[9]

Structure

eIF1 is a conserved translation protein in all eukaryotic cells that is responsible for the investigation of codon-anticodon mismatches during the initiation of translation. In order to determine the structure of human eIF1, an experiment with N-terminal His tag and eIF1 are conducted via using NMR spectroscopy. Scientists have discovered a binding site by generating yeast mutation and study the neighbor conserved residues located in the same region. GST pull-down experiments has shown that eIF1 binds precisely to the p110 subunit of eIF3 as a result explaining eIF1 recruiting. ^[10]

Function

The function of eIf1 has some hidden aspects. However, in all eukaryotic cells initiation of mRNA translation starts with scanning via ribosomal 43S preinitiation complexes starting from the 5’ end of the mRNA. Next, induction via eIF1 and eIF1A are needed to disclose the conformation of the 40S subunit in order to induce DEAD-box RNA helicase eIF4A, its cofactor eIF4B, and eIF4G activity.^[11]

See also

Eukaryotic initiation factors^[12]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000173812 – Ensembl, May 2017

^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000035530 – Ensembl, May 2017

^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

^ Fields C, Adams MD (January 1994). "Expressed sequence tags identify a human isolog of the suil translation initiation factor". Biochemical and Biophysical Research Communications. 198 (1): 288–91. doi:10.1006/bbrc.1994.1040. PMID 7904817.

^ Sheikh MS, Fernandez-Salas E, Yu M, Hussain A, Dinman JD, Peltz SW, Huang Y, Fornace AJ (June 1999). "Cloning and characterization of a human genotoxic and endoplasmic reticulum stress-inducible cDNA that encodes translation initiation factor 1(eIF1(A121/SUI1))". The Journal of Biological Chemistry. 274 (23): 16487–93. doi:10.1074/jbc.274.23.16487. PMID 10347211.

^ "Entrez Gene: EIF1 eukaryotic translation initiation factor 1".

^ ^a ^b Aitken CE, Lorsch JR (June 2012). "A mechanistic overview of translation initiation in eukaryotes". Nature Structural & Molecular Biology. 19 (6): 568–76. doi:10.1038/nsmb.2303. PMID 22664984. S2CID 9201095.

^ Fraser CS (July 2015). "Quantitative studies of mRNA recruitment to the eukaryotic ribosome". Biochimie. 114: 58–71. doi:10.1016/j.biochi.2015.02.017. PMC 4458453. PMID 25742741.

^ Fletcher CM, Pestova TV, Hellen CU, Wagner G (May 1999). "Structure and interactions of the translation initiation factor eIF1". The EMBO Journal. 18 (9): 2631–7. doi:10.1093/emboj/18.9.2631. PMC 1171342. PMID 10228174.

^ Jackson RJ, Hellen CU, Pestova TV (February 2010). "The mechanism of eukaryotic translation initiation and principles of its regulation". Nature Reviews. Molecular Cell Biology. 11 (2): 113–27. doi:10.1038/nrm2838. PMC 4461372. PMID 20094052.

^ "EIF1 - Eukaryotic translation initiation factor 1 - Homo sapiens (Human) - EIF1 gene & protein". www.uniprot.org. Retrieved 2018-09-23.

Further reading

Lian Z, Pan J, Liu J, Zhang S, Zhu M, Arbuthnot P, Kew M, Feitelson MA (March 1999). "The translation initiation factor, hu-Sui1 may be a target of hepatitis B X antigen in hepatocarcinogenesis". Oncogene. 18 (9): 1677–87. doi:10.1038/sj.onc.1202470. PMID 10208429. S2CID 22809502.

Fletcher CM, Pestova TV, Hellen CU, Wagner G (May 1999). "Structure and interactions of the translation initiation factor eIF1". The EMBO Journal. 18 (9): 2631–7. doi:10.1093/emboj/18.9.2631. PMC 1171342. PMID 10228174.

Chin LS, Singh SK, Wang Q, Murray SF (2000). "Identification of okadaic-acid-induced genes by mRNA differential display in glioma cells". Journal of Biomedical Science. 7 (2): 152–9. doi:10.1007/BF02256622. PMID 10754390.

Mendell JT, Medghalchi SM, Lake RG, Noensie EN, Dietz HC (December 2000). "Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes". Molecular and Cellular Biology. 20 (23): 8944–57. doi:10.1128/MCB.20.23.8944-8957.2000. PMC 86549. PMID 11073994.

Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (January 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.

External links

EIF1 human gene location in the UCSC Genome Browser.
EIF1 human gene details in the UCSC Genome Browser.
Cap-dependent translation initiation from Nature Reviews Microbiology. A good image and overview of the function of initiation factors
Overview of all the structural information available in the PDB for UniProt: P41567 (Eukaryotic translation initiation factor 1) at the PDBe-KB.

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