The women's 4x400 metres relay event at the 2000 World Junior Championships in Athletics was held in Santiago, Chile, at Estadio Nacional Julio Martínez Prádanos on 21 and 22 October.[1][2]
22 October
21 October
| Rank | Nation | Competitors | Time | Notes |
|---|---|---|---|---|
| 1 |  Germany |
Eileen Müller Claudia Hoffmann Nadine Balkow Larissa Kettenis |
3:38.18 | Q |
| 2 |  United Kingdom |
Heather Brookes Jenny Meadows Helen Thieme Lisa Miller |
3:38.38 | Q |
| 3 |  Ukraine |
Svetlana Chervan Tatyana Petlyuk Antonina Yefremova Natalya Pygyda |
3:38.92 | Q |
 Botswana |
Ramomene Boikhutso Geoitsemodimo Dikinya Ethel Masoloko Orapeleng Keboifile |
DQ | ||
 Colombia |
Sira Córdoba Rosibel García Melisa Murillo Norma González |
DQ |
| Rank | Nation | Competitors | Time | Notes |
|---|---|---|---|---|
| 1 |  Jamaica |
Kashain Page Sheryl Morgan Anneisha McLaughlin Shellene Williams |
3:37.38 | Q |
| 2 |  Romania |
Adela Marchis Liliana Barbulescu Maria Rus Alina Rîpanu |
3:37.65 | Q |
| 3 |  Poland |
Dorota Wojtczak Monika Bejnar Dorota Dydo Aneta Lemiesz |
3:41.24 | Q |
| 4 |  Australia |
Lauren Jauncey Caitlin Willis Rebecca Irwin Katerina Dressler |
3:42.45 | q |
| 5 |  New Zealand |
Rachael Signal April Brough Anna Spriggens Monique Williams |
3:46.02 | q |
According to an unofficial count, 45 athletes from 10 countries participated in the event.
Australia (5) Botswana (4) Colombia (4) Germany (4) Jamaica (6) New Zealand (4) Poland (5) Romania (4) Ukraine (4) United Kingdom (5)
