The women's 4 × 400 metres relay at the 2009 World Championships in Athletics was held at the Olympic Stadium on August 22 and August 23.
* Runners who participated in the heats only and received medals.
**Original medalists Russia forfeited results and medals as a result of disqualification of Kapachinskaya[1].
Prior to the competition, the following records were as follows.
No new world or championship record was set during this competition.
| Standard |
|---|
| 3:31.00 |
| Date | Time | Round |
|---|---|---|
| August 22, 2009 | 20:15 | Heats |
| August 23, 2009 | 17:50 | Final |
Qualification: First 3 of each heat (Q) plus the 2 fastest times (q) advance to the final.
Key: DQ = Disqualified, Q = qualification by place in heat, q = qualification by overall place, SB = Seasonal best
| Rank | Nation | Athletes | Time | Notes |
|---|---|---|---|---|
|
 United States (USA)
|
Debbie Dunn, Allyson Felix, Lashinda Demus, Sanya Richards | 3:17.83 | WL |
|
 Jamaica (JAM)
|
Rosemarie Whyte, Novlene Williams-Mills, Shereefa Lloyd, Shericka Williams | 3:21.15 | SB |
 Russia (RUS)
|
Anastasiya Kapachinskaya, Tatyana Firova, Lyudmila Litvinova, Antonina Krivoshapka | Doping | ||
|
 Great Britain & N.I. (GBR)
|
Lee McConnell, Christine Ohuruogu, Vicki Barr, Nicola Sanders | 3:25.16 | SB[2] |
| 4 |  Germany (GER)
|
Fabienne Kohlmann, Sorina Nwachukwu, Esther Cremer, Claudia Hoffmann | 3:27.61 | |
| 5 |  Nigeria (NGR)
|
Endurance Abinuwa, Muizat Ajoke Odumosu, Josephine Ehigie, Folasade Abugan | 3:28.55 | SB |
| 6 |  France (FRA)
|
Virginie Michanol, Aurélie Kamga, Symphora Béhi, Solen Désert-Mariller | 3:30.16 | |
| 7 |  Cuba (CUB)
|
Diosmely Peña, Daisurami Bonne, Zulia Calatayud, Indira Terrero | 3:36.99 |
Key: SB = Seasonal best, WL = World leading (in a given season)
