The DRN issues serotonergic efferents to the hippocampal formation, limbic lobe, and amygdala (these efferents are involved in regulation of memory processing).[2]
The dorsal raphe is the largest serotonergic nucleus and provides a substantial proportion of the serotonin innervation to the forebrain.
Serotonergic neurons are found throughout the dorsal raphe nucleus and tend to be larger than other cells. A substantial population of cells synthesizing substance P are found in the rostral aspects, many of these co-express serotonin and substance P. There is also a population of catecholamine synthesizing neurons in the rostral dorsal raphe, and these cells appear to be relatively large.[3]
The dorsal raphe nucleus has also been implicated in naloxone-induced morphine withdrawal. It is known that endogenous opioid receptors exist in the dorsal raphe nucleus, and that it is a focal point as an ascending and descending regulator. Pourshanazari et al. showed in their 2000 paper that electrical stimulation of the dorsal raphe nucleus can partially alleviate morphine withdrawal symptoms via electrical stimulation of the raphe nucleus in question.[5]
These are fascinating results; however no control was provided for the spread of electrical charge to other parts of the brain stem. It is quite possible that the charge spread to the nucleus raphes magnus and induced analgesia upon the rats. Knowing that the spread of charge across such a short area is very plausible, as is an alternate connection to the raphe magnus, these results could be called into question.
Wu M.F. et al. studied the dorsal raphe nucleus as it pertained to narcolepsy. This is logical, as the raphe nuclei have been known to play a role in the sleep/wake cycle. Cataplexy is the symptom of narcolepsy when full awareness of the environment is maintained, but all muscle tone is lost. This has thought to be a dissociation of what normally happens during REM sleep, when all muscle tone is lost except for the eyes. The dorsal raphe nucleus has been known to project to the lateral hypothalamus, along with the locus coeruleus and the tuberomammillary nucleus. The neurotransmitters of these three aforementioned nuclei, which project to the lateral hypothalamus, are serotonin, norepinephrine and histamine respectively. These neurotransmitters are fully active during waking hours, partially active during non-REM sleep, and have almost ceased during REM sleep. In cats with pontine lesions, their normal atonia is not present, the dorsal raphe nucleus is fully active, as opposed to the cessation of action under normal conditions. A muscle relaxant, known as Mephenesin, reduces activity of the dorsal nucleus, as well as microinjections of carbachol (which induces atonia while awake).[6]
The rostral raphe nuclei, both the median raphe nucleus and particularly the dorsal raphe nucleus have long been implicated in depression. Some studies have suggested that the dorsal raphe may be decreased in size in people with depression and, paradoxically, an increased cell density in those who die by suicide.[7][8][9]
Ten percent of the axons from the dorsal raphe nucleus of the rat have been shown to project to the amygdala,[10] while only medium cells seem to project to the caudate and putamen and olfactory bulb.[11][12]
^O'Hearn E, Molliver ME (December 1984). "Organization of raphe-cortical projections in rat: a quantitative retrograde study". Brain Res. Bull. 13 (6): 709–26. doi:10.1016/0361-9230(84)90232-6. PMID6099744. S2CID4761755.
^Patestas, Maria A.; Gartner, Leslie P. (2016). A Textbook of Neuroanatomy (2nd ed.). Hoboken, New Jersey: Wiley-Blackwell. p. 432. ISBN978-1-118-67746-9.
^Baker KG, Halliday GM, Hornung JP, Geffen LB, Cotton RG, Törk I (1991). "Distribution, morphology and number of monoamine-synthesizing and substance P-containing neurons in the human dorsal raphe nucleus". Neuroscience. 42 (3): 757–75. doi:10.1016/0306-4522(91)90043-N. PMID1720227. S2CID23034680.
^Briley M, Moret C (October 1993). "Neurobiological mechanisms involved in antidepressant therapies". Clin Neuropharmacol. 16 (5): 387–400. doi:10.1097/00002826-199310000-00002. PMID8221701.
^Pourshanazari, A.A.; Alaei; Rafati (2000). "Effects of Electrical Stimulation of Nucleus Raphe Dorsalis on initiation of morphine self-administration in rats". Medical Journal of Islamic Academy of Sciences. 13 (2): 63–7.
^Ma QP, Yin GF, Ai MK, Han JS (December 1991). "Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat". Neurosci. Lett. 134 (1): 21–4. doi:10.1016/0304-3940(91)90499-J. PMID1815148. S2CID5713957.
^Steinbusch HW, Nieuwenhuys R, Verhofstad AA, Van der Kooy D (1981). "The nucleus raphe dorsalis of the rat and its projection upon the caudatoputamen. A combined cytoarchitectonic, immunohistochemical and retrograde transport study". J. Physiol. (Paris). 77 (2–3): 157–74. PMID6169825.
^Petzold GC, Hagiwara A, Murthy VN (June 2009). "Serotonergic modulation of odor input to the mammalian olfactory bulb". Nat. Neurosci. 12 (6): 784–91. doi:10.1038/nn.2335. PMID19430472. S2CID33863055.
