Inbiochemistry, isozymes (also known as isoenzymes or more generally as multiple forms of enzymes) are enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes usually have different kinetic parameters (e.g. different KM values), or are regulated differently. They permit the fine-tuning of metabolism to meet the particular needs of a given tissue or developmental stage.
In many cases, isozymes are encoded by homologous genes that have diverged over time. Strictly speaking, enzymes with different amino acid sequences that catalyse the same reaction are isozymes if encoded by different genes, or allozymes if encoded by different alleles of the same gene; the two terms are often used interchangeably.
Isozymes were first described by R. L. Hunter and Clement Markert (1957) who defined them as different variants of the same enzyme having identical functions and present in the same individual.[1] This definition encompasses (1) enzyme variants that are the product of different genes and thus represent different loci (described as isozymes) and (2) enzymes that are the product of different alleles of the same gene (described as allozymes).[2]
Isozymes are usually the result of gene duplication, but can also arise from polyploidisationornucleic acid hybridization. Over evolutionary time, if the function of the new variant remains identical to the original, then it is likely that one or the other will be lost as mutations accumulate, resulting in a pseudogene. However, if the mutations do not immediately prevent the enzyme from functioning, but instead modify either its function, or its pattern of expression, then the two variants may both be favoured by natural selection and become specialised to different functions.[3] For example, they may be expressed at different stages of development or in different tissues.[4]
Allozymes may result from point mutations or from insertion-deletion (indel) events that affect the coding sequence of the gene. As with any other new mutations, there are three things that may happen to a new allozyme:
An example of an isozyme is glucokinase, a variant of hexokinase which is not inhibited by glucose 6-phosphate. Its different regulatory features and lower affinity for glucose (compared to other hexokinases), allow it to serve different functions in cells of specific organs, such as control of insulin release by the beta cells of the pancreas, or initiation of glycogen synthesis by liver cells. Both these processes must only occur when glucose is abundant.
1.) The enzyme lactate dehydrogenase is a tetramer made of two different sub-units, the H-form and the M-form. These combine in different combinations depending on the tissue:[7]
| Type | Composition | Location | Electrophoretic Mobility | Whether destroyed by
Heat (at 60 °C) |
Percentage of normal
serum in humans |
|---|---|---|---|---|---|
| LDH1 | HHHH | Heart and Erythrocyte | Fastest | No | 25% |
| LDH2 | HHHM | Heart and Erythrocyte | Faster | No | 35% |
| LDH3 | HHMM | Brain and Kidney | Fast | Partially | 27% |
| LDH4 | HMMM | Skeletal Muscle and Liver | Slow | Yes | 8% |
| LDH5 | MMMM | Skeletal Muscle and Liver | Slowest | Yes | 5% |
2.) Isoenzymes of creatine phosphokinase:[7] Creatine kinase (CK) or creatine phosphokinase (CPK) catalyses the interconversion of phospho creatine to creatine .
CPK exists in 3 isoenzymes. Each isoenzymes is a dimer of 2 subunits M (muscle), B (brain) or both[7]
| Isoenzyme | Subunit | Tissue of Origin |
|---|---|---|
| CPK1 | BB | Brain |
| CPK2 | MB | Heart |
| CPK3 | MM | Skeletal muscle |
3.) Isoenzymes of alkaline phosphatase:[7] Six isoenzymes have been identified. The enzyme is a monomer, the isoenzymes are due to the differences in the carbohydrate content (sialic acid residues). The most important ALP isoenzymes are α1-ALP, α2-heat labile ALP, α2-heat stable ALP, pre-β ALP and γ-ALP. Increase in α2-heat labile ALP suggests hepatitis whereas pre-β ALP indicates bone diseases.
Isozymes (and allozymes) are variants of the same enzyme. Unless they are identical in their biochemical properties, for example their substrates and enzyme kinetics, they may be distinguished by a biochemical assay. However, such differences are usually subtle, particularly between allozymes which are often neutral variants. This subtlety is to be expected, because two enzymes that differ significantly in their function are unlikely to have been identified as isozymes.
While isozymes may be almost identical in function, they may differ in other ways. In particular, amino acid substitutions that change the electric charge of the enzyme are simple to identify by gel electrophoresis, and this forms the basis for the use of isozymes as molecular markers. To identify isozymes, a crude protein extract is made by grinding animal or plant tissue with an extraction buffer, and the components of extract are separated according to their charge by gel electrophoresis. Historically, this has usually been done using gels made from potato starch, but acrylamide gels provide better resolution.
All the proteins from the tissue are present in the gel, so that individual enzymes must be identified using an assay that links their function to a staining reaction. For example, detection can be based on the localised precipitation of soluble indicator dyes such as tetrazolium salts which become insoluble when they are reducedbycofactors such as NADorNADP, which generated in zones of enzyme activity. This assay method requires that the enzymes are still functional after separation (native gel electrophoresis), and provides the greatest challenge to using isozymes as a laboratory technique.
Isoenzymes differ in kinetics (they have different KM and Vmax values).
Population genetics is essentially a study of the causes and effects of genetic variation within and between populations, and in the past, isozymes have been amongst the most widely used molecular markers for this purpose. Although they have now been largely superseded by more informative DNA-based approaches (such as direct DNA sequencing, single nucleotide polymorphisms and microsatellites), they are still among the quickest and cheapest marker systems to develop, and remain (as of 2005[update]) an excellent choice for projects that only need to identify low levels of genetic variation, e.g. quantifying mating systems.
Authority control databases: National  |
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