Sambasiva Iyer was born in 1888 in Tirugokarnam, Pudukottai district as the second son to Veena Vidwan Subbiah Iyer. Sambasiva Iyer learnt Veena from his father along with his elder brother Subbarama Iyer. The duo belonged to the seventh generation to carry the family's high unbroken Veena tradition. The two brothers played together as "Karaikudi brothers" and enjoyed an unbroken career from their debut in their teens to the year 1934. Sambasiva Iyer was known for his tremendous hard work or "Asura Sadhaka". His mastery over the instrument was perfect and he constantly toiled to preserve the purity of knowledge he obtained from his ancestors.
Sambasiva Iyer did not have any children. However, as he lived in patriarchal society, he found it necessary to pass on the family legacy through a male member of the family. In 1957, he adopted Karaikudi S. Subramanian, who was the son of Subbarama Iyer's 3rd daughter Lakshmi Ammal.
Amongst his noted disciples are Kalaimamani and President's award winner (late) Ranganayaki Rajagopalan, Rajeshwari Padmanabhan, Jayalakshmi Sugumar and Karaikudi Subramhanian.[5]
Karaikudi Sambasiva Iyer is not to be confused with his namesake musician Padinaindumandapa Sambasiva Iyer. PSI was much older, and guru to two recipients of Sangeet Kalanidhi award : Muthiah Bhagavatar, and his (PSI's) son Sabhesha (सभेश) Iyer.