This is a list of members of the fourth parliament of the South Australian House of Assembly, which sat from 31 March 1865 until 26 March 1868. The members were elected at the 1865 colonial election.
| Name | Electorate | Term in Office |
|---|---|---|
| Richard Andrews | The Sturt | 1857–1860, 1862–1870 |
| Hon Arthur Blyth | Gumeracha | 1857–1868, 1870–1877 |
| Neville Blyth [9] | East Torrens | 1860–1867, 1868–1870, 1871, 1877–1878 |
| James Boucaut | West Adelaide | 1861–1862, 1865–1870, 1871–1878 |
| Henry Bright | Stanley | 1865–1884 |
| David Bower | Port Adelaide | 1865–1870, 1875–1887 |
| John Carr [1] | Noarlunga | 1865–1879, 1881–1884 |
| Wentworth Cavenagh | Yatala | 1862–1875, 1875–1881 |
| Patrick Coglin | Light | 1860–1868, 1870–1871, 1875–1881, 1882–1887 |
| George William Cole [5] | The Burra | 1860–1866 |
| John Colton | Noarlunga | 1862–1870, 1875–1878, 1880–1887 |
| Walter Duffield | Barossa | 1857–1868, 1870–1871 |
| John Dunn | Mount Barker | 1857–1868, 1868 |
| Francis Dutton [2] | Light | 1857–1862, 1862–1865 |
| William Everard | Encounter Bay | 1865–1870, 1871–1872 |
| Daniel Fisher [9] | East Torrens | 1867–1870 |
| Henry Fuller | West Adelaide | 1865–1870 |
| Lavington Glyde | Yatala | 1857–1875, 1877–1884 |
| Charles Goode [7] | East Torrens | 1865–1866 |
| Adam Lindsay Gordon [6] | Victoria | 1865–1866 |
| Alexander Hay [8] | Gumeracha | 1857–1861, 1867–1871 |
| John Hart [3] | Port Adelaide | 1857–1859, 1862–1866, 1868–1873 |
| George Kingston | Stanley | 1857–1860, 1861–1880 |
| James Martin | Barossa | 1865–1868 |
| Alexander McCulloch [5] | The Burra | 1866–1868 |
| William Milne | Onkaparinga | 1857–1868 |
| Alexander Borthwick Murray [8] | Gumeracha | 1862–1867 |
| Alexander Murray [10] | The Sturt | 1867–1868 |
| John Bentham Neales | The Burra | 1857–1860, 1862–1870 |
| Joseph Peacock [10] | The Sturt | 1860–1867 |
| John Pickering | West Torrens | 1865–1868, 1870, 1871–1878 |
| James Rankine | Mount Barker | 1865–1868 |
| Hon Thomas Reynolds | East Adelaide | 1857–1862, 1862, 1864–1870, 1871–1872, 1872–1873 |
| John Riddoch | Victoria | 1865–1870, 1871–1873 |
| John Rounsevell [2] | Light | 1865–1868, 1880–1881 |
| Philip Santo | East Adelaide | 1860–1870 |
| Jacob Smith [3] | Port Adelaide | 1866–1868 |
| Augustine Stow [4] | Flinders | 1862–1865, 1866–1868 |
| Randolph Isham Stow [7] | East Torrens | 1861–1865, 1866–1868, 1873–1875 |
| Hon Henry Strangways | West Torrens | 1858–1871 |
| David Sutherland | Encounter Bay | 1860–1862, 1862–1868 |
| William Townsend | Onkaparinga | 1857–1882 |
| William Trimmer [1] | Noarlunga | 1865 |
| James Umpherston [6] | Victoria | 1866–1868 |
| Alfred Watts [4] | Flinders | 1862–1866, 1868–1875 |
| John Williams | Flinders | 1864–1868, 1875–1878 |
