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a
Techniques used to study nucleic acids
For protein methods, see
Protein methods
.
Nucleic acid methods
are the techniques used to study
nucleic acids
:
DNA
and
RNA
.
Purification
[
edit
]
DNA extraction
Phenol–chloroform extraction
Minicolumn purification
RNA extraction
Boom method
Synchronous coefficient of drag alteration
(SCODA) DNA purification
Quantification
[
edit
]
Abundance in weight: spectroscopic
nucleic acid quantitation
Absolute abundance in number:
real-time polymerase chain reaction
(quantitative PCR)
High-throughput relative abundance:
DNA microarray
High-throughput absolute abundance:
serial analysis of gene expression
(SAGE)
Size:
gel electrophoresis
Synthesis
[
edit
]
De novo
:
oligonucleotide synthesis
Amplification:
polymerase chain reaction
(PCR)
Kinetics
[
edit
]
Multi-parametric surface plasmon resonance
[1]
[2]
Dual-polarization interferometry
[3]
Quartz crystal microbalance with dissipation monitoring
(QCM-D)
[4]
Gene function
[
edit
]
RNA interference
[5]
Other
[
edit
]
Bisulfite sequencing
DNA sequencing
Expression cloning
Fluorescence in situ hybridization
Lab-on-a-chip
Comparison of nucleic acid simulation software
Northern blot
Nuclear run-on
assay
Radioactivity in the life sciences
Southern blot
Differential centrifugation
(sucrose gradient)
Toeprinting assay
Several
bioinformatics
methods, as seen in
list of RNA structure prediction software
See also
[
edit
]
CSH Protocols
Current Protocols
References
[
edit
]
^
Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations".
Chem. Commun
.
50
(
92
): 14352–14355.
doi
:
10.1039/C4CC07041K
.
PMID
25295484
.
^
Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014).
"An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes"
.
Nanotechnology
.
25
(
9
): 094009.
Bibcode
:
2014Nanot..25i4009I
.
doi
:
10.1088/0957-4484/25/9/094009
.
PMID
24522116
.
S2CID
206070778
.
^
Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation".
Biosensors and Bioelectronics
.
21
(
4
): 618–626.
doi
:
10.1016/j.bios.2004.12.024
.
PMID
16202875
.
^
Dixon, Matthew C. (July 2008).
"Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions"
.
Journal of Biomolecular Techniques
.
19
(
3
): 151–158.
PMC
2563918
.
PMID
19137101
.
^
Hannon, Gregory J. (July 2002).
"RNA interference"
.
Nature
.
418
(6894): 244–251.
Bibcode
:
2002Natur.418..244H
.
doi
:
10.1038/418244a
.
ISSN
1476-4687
.
PMID
12110901
.
S2CID
4426281
.
External links
[
edit
]
Protocols for Recombinant DNA Isolation, Cloning, and Sequencing
v
t
e
Molecular biology
History
Index
Glossary
Overview
Central dogma
DNA replication
(
DNA
)
Transcription
(
RNA
)
Translation
(
protein
)
Element
Genetic
Heredity
Promoter
Pribnow box
TATA box
Operon
gal operon
lac operon
trp operon
Intron
Exon
Terminator
Enhancer
Repressor
lac repressor
trp repressor
Silencer
Histone methylation
Linked life
Cell biology
Biochemistry
Computational biology
Developmental biology
Functional biology/medicine
Genetics
Engineering
Concepts
Cultured meat
Mitosis
Cell signalling
Post-transcriptional modification
Post-translational modification
Dry lab
/
Wet lab
Techniques
Cell culture
Model organisms
(such as
C57BL/6 mice
)
Methods
Nucleic acid
Protein
Fluorescence
,
Pigment
&
Radioactivity
High-throughput technique ("
-omics
")
DNA microarray
Mass spectrometry
Lab-on-a-chip
Gene regulation
Epigenetic
Genetic
Post-transcriptional
Post-translational regulation
Molecular biology
WikiProject
R
e
t
r
i
e
v
e
d
f
r
o
m
"
https://en.wikipedia.org/w/index.php?title=Nucleic_acid_methods&oldid=1187433882
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