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Reports of Streptococcus mitis on the Moon: Difference between revisions







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{{Short description|Bacteria found on Surveyor 3 lunar probe}}

[[Image:Apollo12ConradSurveyor.jpg|thumb|right|200px|[[Pete Conrad]] with Surveyor 3]]

[[Image:Apollo12ConradSurveyor.jpg|thumb|right|200px|[[Pete Conrad]] with Surveyor 3]]

As part of the [[Apollo 12]] mission, the camera from the [[Surveyor 3]] probe was brought back from the [[Moon]] to Earth. On analyzing the camera it was found that the common bacterium ''[[Streptococcus mitis]]'' was alive on the camera. This was attributed by NASA to the camera not being sterilized on Earth prior to its launch two and a half years previously.{{citation needed|reason=Your explanation here|date=June 2015}}

As part of the [[Apollo 12]] mission in November 1969, the camera from the [[Surveyor 3]] probe was brought back from the [[Moon]] to Earth. On analyzing the camera, it was found that the common bacterium ''[[Streptococcus mitis]]'' was alive on the camera. [[NASA]] reasoned that the camera was not [[Sterilization (microbiology)|sterilize]]d on Earth before the [[space probe]]'s launch in April 1967, two and a half years earlier.<ref name="NASA" /> However, later study showed that the scientists analysing the camera on return to Earth used procedures that were inadequate to prevent [[recontamination]] after return to Earth, for instance with their arms exposed, not covering their entire bodies as modern scientists would do. There may also have been possibilities for [[contamination]] during the return mission as the camera was returned in a porous bag rather than the airtight containers used for lunar [[Sample (material)|sample]] return.<ref name="David2011" /> As a result, the source of the contamination remains controversial.



==History==

==History==

Since the Apollo Program, there has been at least one independent investigation into the validity of the NASA claim. [[Leonard D. Jaffe]], a [[Surveyor program]] scientist and custodian of the Surveyor 3 parts brought back from the Moon, stated in a letter to the [[Planetary Society]] that a member of his staff reported that a "breach of sterile procedure" took place at just the right time to produce a [[false positive]] result. One of the implements being used to scrape samples off the Surveyor parts was laid down on a non-sterile laboratory bench, and then was used to collect surface samples for culturing. Jaffe wrote, "It is, therefore, quite possible that the microorganisms were transferred to the camera after its return to Earth, and that they had never been to the Moon."<ref>{{cite web|url=http://www.astrobio.net/news/article1311.html|title=Apollo 12 Remembered. Lunar Germ Colony or Lab Anomaly?|archiveurl=https://web.archive.org/web/20060831132850/http://www.astrobio.net/news/article1311.html|archivedate=2006-08-31|deadurl=yes|date=2004-11-21|work=Astrobiology Magazine}}</ref> In 2007, [[NASA]] funded an archival study that sought the film of the camera-body microbial sampling, to confirm the report of a breach in sterile technique.

Since the Apollo Program, there has been at least one independent investigation into the validity of the NASA claim. [[Leonard D. Jaffe]], a [[Surveyor program]] scientist and custodian of the Surveyor 3 [[Surveyor 3#Retrieved parts|parts]] brought back from the Moon, stated in a letter to the [[Planetary Society]] that a member of his staff reported that a "breach of sterile procedure" took place at just the right time to produce a [[false positive]] result. One of the implements being used to scrape samples off the Surveyor parts was laid down on a non-sterile laboratory bench, and then was used to collect surface samples for culturing.Jaffe wrote, "It is, therefore, quite possible that the microorganisms were transferred to the camera after its return to Earth, and that they had never been to the Moon."<ref>{{cite magazine|url=http://www.astrobio.net/news/article1311.html|title=Apollo 12 Remembered. Lunar Germ Colony or Lab Anomaly?|archive-url=https://web.archive.org/web/20060831132850/http://www.astrobio.net/news/article1311.html|archivedate=2006-08-31|url-status=dead|date=2004-11-21|work=Astrobiology Magazine}}</ref> In 2007, [[NASA]] funded an archival study that sought the film of the camera-body microbial sampling, to confirm the report of a breach in sterile technique.



The bacterial test is now non-repeatable because the parts were subsequently taken out of quarantine and fully re-exposed to terrestrial conditions (the Surveyor 3 camera is now on display in the [[Smithsonian Air and Space Museum]] in [[Washington, D.C.]]).

The bacterial test is now non-repeatable because the parts were subsequently taken out of quarantine and fully re-exposed to terrestrial conditions (the Surveyor 3 camera is now on display in the [[Smithsonian Air and Space Museum]] in [[Washington, D.C.]]).



The Surveyor 3 camera was returned from the Moon in a nylon [[duffel bag]], and was not in the type of sealed airtight metal container used to return lunar samples in the early Apollo missions. It is therefore possible that it was contaminated by the astronauts and the environment in the Apollo 12 capsule itself.<ref>{{cite web |url=http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651 |title=LSDA - Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT) |accessdate=2011-05-15 |work=Life Sciences Data Archive |publisher=[[National Aeronautics and Space Administration]] |archiveurl=https://web.archive.org/web/20100619225547/http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651 |archivedate=2010-06-19 |deadurl=yes}}</ref> 

The Surveyor 3 camera was returned from the Moon in a nylon [[duffel bag]], and was not in the type of sealed airtight metal container used to return lunar samples in the early Apollo missions. It is therefore possible that it was contaminated by the astronauts and the environment in the Apollo 12 capsule itself.<ref name="NASA">{{cite web |url=http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651 |title=LSDA Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT) |accessdate=2011-05-15 |work=Life Sciences Data Archive |publisher=[[National Aeronautics and Space Administration]] |archiveurl=https://web.archive.org/web/20100619225547/http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651 |archivedate=2010-06-19 |url-status=dead}}</ref> 



In March 2011, three researchers co-authored a paper, entitled "A Microbe on the Moon? Surveyor III and Lessons Learned for Future Sample Return Missions" that assessed the validity of claims that the ''S. mitis'' samples found on the camera had indeed survived for nearly three years on the Moon. The paper concluded that the presence of microbes could more likely be attributed to poor [[clean room]] conditions rather than the survival of bacteria for three years in the harsh moon environment. The paper also discussed the implication this incident would have for contamination control in future space missions.<ref name="David">{{cite news |first=Leonard |last=David |title=Moon Microbe Mystery Finally Solved |date=2 May 2011 |url=http://www.space.com/11536-moon-microbe-mystery-solved-apollo-12.html |work=[[Space.com]] |accessdate=2011-05-15}}</ref>

In March 2011, three researchers co-authored a paper titled "A Microbe on the Moon? Surveyor III and Lessons Learned for Future Sample Return Missions" that assessed the validity of claims that the ''S. mitis'' samples found on the camera had indeed survived for nearly three years on the Moon. The paper concluded that the presence of microbes could more likely be attributed to poor [[clean room]] conditions rather than the survival of bacteria for three years in the harsh environment of the Moon. The paper also discussed the implication this incident would have for contamination control in future space missions.<ref name="David2011">{{cite news |first=Leonard |last=David |title=Moon Microbe Mystery Finally Solved |date=2 May 2011 |url=http://www.space.com/11536-moon-microbe-mystery-solved-apollo-12.html |work=[[Space.com]] |accessdate=2011-05-15}}</ref><ref>{{cite web|last1=John D. Rummel, Judith H. Allton, and Don Morrison|title=A Microbe on the Moon? Surveyor III and Lessons Learned for Future Sample Return Missions |url=http://www.lpi.usra.edu/meetings/sssr2011/pdf/5023.pdf|location=Solar System Sample Return Mission (2011)|date=2011}}</ref>



Countervailing evidence against the secondary contamination hypothesis is the fact that, according to Lieutenant Colonel Fred Mitchell, lead author of the original 1971 paper<ref name="Mitchell">Mitchell, F. J., & Ellis, W. L. (1971). [http://adsabs.harvard.edu/full/1971LPSC....2.2721M Surveyor III: Bacterium isolated from lunar retrieved TV camera]. In A.A. Levinson (ed.). Proceedings of the second lunar science conference. MIT Press, Cambridge.</ref> there was a significant delay before the sampled culture began growing: this is consistent with the sampled bacteria consisting of dormant spores, but not if the sampled culture was the result of fresh contamination. In addition, according to Mitchell, the microbes clung exclusively to the foam during culturing, which would not have happened had there been contamination.<ref name = "David" /> Furthermore, if fresh contamination had occurred, millions of individual bacteria and "a representation of the entire microbial population would be expected"; instead, only a few individual bacteria were sampled, and only from a single species.<ref name="Mitchell" />

Countervailing evidence against the [[secondary contamination]] hypothesis is the fact that, according to Lieutenant Colonel Fred Mitchell, lead author of the original 1971 paper,<ref name="Mitchell-Ellis 1971">{{cite journal|last=Mitchell |first=F. J. |last2=Ellis |first2=W. L. |date=1971 |url=http://adsabs.harvard.edu/full/1971LPSC....2.2721M |title=Surveyor III: Bacterium isolated from lunar retrieved TV camera |editor-first=A. A. |editor-last=Levinson |journal=Proceedings of the second lunar science conference |publisher=MIT Press |location=Cambridge}}</ref> there was a significant delay before the sampled culture began growing; this is consistent with the sampled bacteria consisting of dormant cells, but not if the sampled culture was the result of fresh contamination. In addition, according to Mitchell, the microbes clung exclusively to the foam during culturing, which would not have happened had there been contamination.<ref name="David2011" /> Furthermore, if fresh contamination had occurred, millions of individual bacteria and "a representation of the entire microbial population would be expected"; instead, only a few individual bacteria were sampled, and only from a single species.<ref name="Mitchell-Ellis 1971" />



This subject was covered in the 2008 Discovery Channel documentary series, ''[[When We Left Earth]]'' and on episode of the [[Science Channel]] series ''[[Nasa's Unexplained Files]]'' entitled ''Return of the Moon Bugs''.

This subject was covered in the 2008 Discovery Channel documentary series, ''[[When We Left Earth]]'' and in the [[Science Channel]] series ''[[Nasa's Unexplained Files]]'' episode ''Return of the Moon Bugs''.



==References==

==References==

Line 19: Line 20:


==External links==

==External links==

*{{cite web|url=http://astrobiology.arc.nasa.gov/news/expandnews.cfm?id=10180}}

* {{cite web|url=http://astrobiology.arc.nasa.gov/news/expandnews.cfm?id=10180|title=Ocean Rendezvous|url-status=dead|archiveurl=https://web.archive.org/web/20060929013731/http://astrobiology.arc.nasa.gov/news/expandnews.cfm?id=10180|archivedate=2006-09-29}}

*{{cite web|title=Apollo 12 Mission|url=http://www.lpi.usra.edu/expmoon/Apollo12/A12_Experiments_III.html|website=www.lpi.usra.edu}}

* {{cite web|title=Apollo 12 Mission|url=http://www.lpi.usra.edu/expmoon/Apollo12/A12_Experiments_III.html|website=www.lpi.usra.edu}}

*{{cite web|title=http://news.yahoo.com/s/space/20110506/sc_space/moonmicrobemysteryfinallysolved|url=http://news.yahoo.com/s/space/20110506/sc_space/moonmicrobemysteryfinallysolved}}

* {{cite web|title=Moon Microbe Mystery Finally Solved|url=https://news.yahoo.com/s/space/20110506/sc_space/moonmicrobemysteryfinallysolved|url-status=dead|archiveurl=https://web.archive.org/web/20110509175054/https://news.yahoo.com/s/space/20110506/sc_space/moonmicrobemysteryfinallysolved|archive-date=2011-05-09}}



{{Include-NASA|url=http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651|article=LSDA - Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT)}}

{{Include-NASA|url=http://lsda.jsc.nasa.gov/scripts/experiment/exper.cfm?exp_index=1651|article=Life Sciences Data Archive (LSDA) Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT)}}

{{DISPLAYTITLE:Reports of ''Streptococcus mitis'' on the Moon}}

{{DISPLAYTITLE:Reports of ''Streptococcus mitis'' on the Moon}}

{{Apollo program|state=collapsed}}



[[Category:Astrobiology]]

[[Category:Astrobiology]]

[[Category:Bacteria]]

[[Category:Bacteria]]

[[Category:Apollo 12]]

[[Category:Moon myths]]

[[Category:Moon myths]]


Latest revision as of 21:37, 28 January 2024

Pete Conrad with Surveyor 3

As part of the Apollo 12 mission in November 1969, the camera from the Surveyor 3 probe was brought back from the Moon to Earth. On analyzing the camera, it was found that the common bacterium Streptococcus mitis was alive on the camera. NASA reasoned that the camera was not sterilized on Earth before the space probe's launch in April 1967, two and a half years earlier.[1] However, later study showed that the scientists analysing the camera on return to Earth used procedures that were inadequate to prevent recontamination after return to Earth, for instance with their arms exposed, not covering their entire bodies as modern scientists would do. There may also have been possibilities for contamination during the return mission as the camera was returned in a porous bag rather than the airtight containers used for lunar sample return.[2] As a result, the source of the contamination remains controversial.

History[edit]

Since the Apollo Program, there has been at least one independent investigation into the validity of the NASA claim. Leonard D. Jaffe, a Surveyor program scientist and custodian of the Surveyor 3 parts brought back from the Moon, stated in a letter to the Planetary Society that a member of his staff reported that a "breach of sterile procedure" took place at just the right time to produce a false positive result. One of the implements being used to scrape samples off the Surveyor parts was laid down on a non-sterile laboratory bench, and then was used to collect surface samples for culturing.Jaffe wrote, "It is, therefore, quite possible that the microorganisms were transferred to the camera after its return to Earth, and that they had never been to the Moon."[3] In 2007, NASA funded an archival study that sought the film of the camera-body microbial sampling, to confirm the report of a breach in sterile technique.

The bacterial test is now non-repeatable because the parts were subsequently taken out of quarantine and fully re-exposed to terrestrial conditions (the Surveyor 3 camera is now on display in the Smithsonian Air and Space MuseuminWashington, D.C.).

The Surveyor 3 camera was returned from the Moon in a nylon duffel bag, and was not in the type of sealed airtight metal container used to return lunar samples in the early Apollo missions. It is therefore possible that it was contaminated by the astronauts and the environment in the Apollo 12 capsule itself.[1]

In March 2011, three researchers co-authored a paper titled "A Microbe on the Moon? Surveyor III and Lessons Learned for Future Sample Return Missions" that assessed the validity of claims that the S. mitis samples found on the camera had indeed survived for nearly three years on the Moon. The paper concluded that the presence of microbes could more likely be attributed to poor clean room conditions rather than the survival of bacteria for three years in the harsh environment of the Moon. The paper also discussed the implication this incident would have for contamination control in future space missions.[2][4]

Countervailing evidence against the secondary contamination hypothesis is the fact that, according to Lieutenant Colonel Fred Mitchell, lead author of the original 1971 paper,[5] there was a significant delay before the sampled culture began growing; this is consistent with the sampled bacteria consisting of dormant cells, but not if the sampled culture was the result of fresh contamination. In addition, according to Mitchell, the microbes clung exclusively to the foam during culturing, which would not have happened had there been contamination.[2] Furthermore, if fresh contamination had occurred, millions of individual bacteria and "a representation of the entire microbial population would be expected"; instead, only a few individual bacteria were sampled, and only from a single species.[5]

This subject was covered in the 2008 Discovery Channel documentary series, When We Left Earth and in the Science Channel series Nasa's Unexplained Files episode Return of the Moon Bugs.

References[edit]

  1. ^ a b "LSDA – Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT)". Life Sciences Data Archive. National Aeronautics and Space Administration. Archived from the original on 2010-06-19. Retrieved 2011-05-15.
  • ^ a b c David, Leonard (2 May 2011). "Moon Microbe Mystery Finally Solved". Space.com. Retrieved 2011-05-15.
  • ^ "Apollo 12 Remembered. Lunar Germ Colony or Lab Anomaly?". Astrobiology Magazine. 2004-11-21. Archived from the original on 2006-08-31.
  • ^ John D. Rummel, Judith H. Allton, and Don Morrison (2011). "A Microbe on the Moon? Surveyor III and Lessons Learned for Future Sample Return Missions" (PDF). Solar System Sample Return Mission (2011).{{cite web}}: CS1 maint: location (link) CS1 maint: multiple names: authors list (link)
  • ^ a b Mitchell, F. J.; Ellis, W. L. (1971). Levinson, A. A. (ed.). "Surveyor III: Bacterium isolated from lunar retrieved TV camera". Proceedings of the second lunar science conference. Cambridge: MIT Press.
  • External links[edit]

    Public Domain This article incorporates public domain material from Life Sciences Data Archive (LSDA) – Experiment: Surveyor 3 Streptococcus Mitis (APSTREPMIT). National Aeronautics and Space Administration.


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