Bunyaviruses belong to the fifth group of the Baltimore classification system, which includes viruses with a negative-sense, single-stranded RNA genome. They have an enveloped, spherical virion. Though generally found in arthropods or rodents, certain viruses in this order occasionally infect humans. Some of them also infect plants.[6] In addition, there is a group of bunyaviruses whose replication is restricted to arthropods and is known as insect-specific bunyaviruses.[7]
A majority of bunyaviruses are vector-borne. With the exception of Hantaviruses and Arenaviruses, all viruses in the Bunyavirales order are transmitted by arthropods (mosquitos, tick, or sandfly). Hantaviruses are transmitted through contact with rodent feces. Incidence of infection is closely linked to vector activity, for example, mosquito-borne viruses are more common in the summer.[6]
Hantaviruses are another medically important member of the order Bunyvirales. They are found worldwide, and are relatively common in Korea, Scandinavia (including Finland), Russia, western North America and parts of South America. Hantavirus infections are associated with high fever, lung edema, and pulmonary failure. The mortality rate varies significantly depending on the form, being up to 50% in New World hantaviruses (the Americas), up to 15% in Old World hantaviruses (Asia and Europe), and as little as 0.1% in Puumala virus (mostly Scandinavia).[9] The antibody reaction plays an important role in decreasing levels of viremia.
Bunyavirus morphology is somewhat similar to that of the Paramyxoviridae family; Bunyavirales form enveloped, spherical virions with diameters of 80–120 nm. These viruses contain no matrix proteins.[10] Instead, the viral surface glycoproteins which form a continuous layer on the virion surface are thought to play a role in the formation of new virions by budding from a cell membrane.[11]
Bunyaviruses have bi- or tripartite genomes consisting of a large (L) and small(s), or large (L), medium (M), and small (S) RNA segment. These RNA segments are single-stranded, and exist in a helical formation within the virion. Besides, they exhibit a pseudo-circular structure due to each segment's complementary ends. The L segment encodes the RNA-dependent RNA polymerase, necessary for viral RNA replication and mRNA synthesis. The M segment encodes the viral glycoproteins, which project from the viral surface and aid the virus in attaching to and entering the host cell. The S segment encodes the nucleocapsid protein (N).[12]
Most bunyaviruses have a negative-sense L and M segment. The S segment of the genus Phlebovirus,[13] and both M and S segment of the genus Tospovirus are ambisense.[14] Ambisense means that some of the genes on the RNA strand are negative sense and others are positive sense. The ambisense S segment codes for the viral nucleoprotein (N) in the negative sense and a nonstructural protein (NSs) in the positive sense. The ambisense M segment codes for glycoprotein (GP) in the negative sense and a nonstructural protein (NSm) in the positive sense.[14]
The total genome size ranges from 10.5 to 22.7 kbp.[15]
The ambisense genome requires two rounds of transcription to be carried out. First, the negative-sense RNA is transcribed to produce mRNA and a full-length replicative intermediate. From this intermediate, a subgenomic mRNA encoding the small segment nonstructural protein is produced while the polymerase produced following the first round of transcription can now replicate the full-length RNA to produce viral genomes.[citation needed]
Bunyaviruses replicate in the cytoplasm, while the viral proteins transit through the ER and Golgi apparatus. Mature virions bud from the Golgi apparatus into vesicles which are transported to the cell surface.[citation needed]
Bunyaviruses infect arthropods, plants, protozoans, and vertebrates.[2] Plants can host bunyaviruses from the families Tospoviridae and Fimoviridae (e.g. tomato, pigeonpea, melon, wheat, raspberry, redbud, and rose). Members of some families are insect-specific, for example the phasmavirids, first isolated from phantom midges,[16] and since identified in diverse insects including moths, wasps and bees, and other true flies.
There are 477 virus species recognised in this order.[1] The phylogenetic tree diagram provides a full list of member species and the hosts which they infect.[2] The order is organized into the following 12 families:[1]
Bunyaviruses have segmented genomes, making them capable of rapid reassortment and increasing the risk of outbreak.[17][18] The bunyavirus that causes severe fever with thrombocytopenia syndrome can undergo recombination both by reassortment of genome segments and by intragenic homologous recombination.[19][20] Bunyaviridae are transmitted by hematophagous arthropods including mosquitoes, midges, flies, and ticks. The viral incubation period is about 48 hours. Symptomatic infection typically causes non-specific flu-like symptoms with fever lasting for about three days. Because of their non-specific symptoms, Bunyavirus infections are frequently mistaken for other illnesses. For example, Bwamba fever is often mistaken for malaria.[21]
Prevention depends on the reservoir, amplifying hosts and how the viruses are transmitted, i.e. the vector, whether ticks or mosquitoes and which animals are involved. Preventive measures include general hygiene, limiting contact with vector saliva, urine, feces, or bedding. There is no licensed vaccine for bunyaviruses. As precautions Cache Valley virus and Hantavirus research are conducted in BSL-2 (or higher), Rift Valley Fever virus research is conducted in BSL-3 (or higher), Congo-Crimean Hemorrhagic Fever virus research is conducted in BSL-4 laboratories.[citation needed]
^"ICTV 9th Report (2011) Bunyaviridae". International Committee on Taxonomy of Viruses (ICTV). Retrieved 31 January 2019. Bunya: from Bunyamwera, place in Uganda, where type virus was isolated.
^Smithburn, K. C.; Haddow, A. J.; Mahaffy, A. F. (March 1946). "A Neurotropic Virus Isolated from Aedes Mosquitoes Caught in the Semliki Forest". The American Journal of Tropical Medicine and Hygiene. s1-26 (2): 189–208. doi:10.4269/ajtmh.1946.s1-26.189. ISSN1476-1645. OCLC677158400. PMID21020339.
^Briese, Thomas; Calisher, Charles H.; Higgs, Stephen (November 2013), "Viruses of the family Bunyaviridae: Are all available isolates reassortants?", Virology, 446 (1–2): 207–216, doi:10.1016/j.virol.2013.07.030, hdl:2097/17679, PMID24074583
^Lv Q, Zhang H, Tian L, Zhang R, Zhang Z, Li J, Tong Y, Fan H, Carr MJ, Shi W. Novel sub-lineages, recombinants and reassortants of severe fever with thrombocytopenia syndrome virus. Ticks Tick Borne Dis. 2017 Mar;8(3):385-390. doi: 10.1016/j.ttbdis.2016.12.015. Epub 2017 Jan 3. PMID28117273
^He CQ, Ding NZ. Discovery of severe fever with thrombocytopenia syndrome bunyavirus strains originating from intragenic recombination. J Virol. 2012 Nov;86(22):12426-30. doi: 10.1128/JVI.01317-12. Epub 2012 Aug 29. PMID22933273
^Patrick R. Murray, Ken S. Rosenthal and Michael A. Pfaller (2008-12-24). Medical Microbiology, 6e (6 ed.). Philadelphia: Mosby. ISBN9780323054706.
