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(Top) 1 Function 2 Interactions 3 References 4 Further reading

Cofactor of BRCA1

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NELFB

Identifiers

Aliases

NELFB, COBRA1, NELF-B, Cofactor of BRCA1, negative elongation factor complex member B

External IDs

OMIM: 611180; MGI: 1931035; HomoloGene: 121600; GeneCards: NELFB; OMA:NELFB - orthologs

Gene location (Human)

Chr.	Chromosome 9 (human)^[1]

Band	9q34.3	Start	137,255,327 bp^[1]
Band	9q34.3	End	137,273,542 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 2 (mouse)^[2]

Band	2\|2 A3	Start	25,089,724 bp^[2]
Band	2\|2 A3	End	25,101,501 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

apex of heart

ventricular zone

right frontal lobe

mucosa of transverse colon

cingulate gyrus

anterior cingulate cortex

ganglionic eminence

amygdala

tendon of biceps brachii

stromal cell of endometrium

Top expressed in

otic vesicle

saccule

medullary collecting duct

mesenteric lymph nodes

molar

otic placode

granulocyte

dentate gyrus of hippocampal formation granule cell

left lung lobe

neural layer of retina

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protein binding RNA binding
Cellular component	nucleus cytoplasm NELF complex nucleoplasm
Biological process	transcription elongation from RNA polymerase II promoter regulation of transcription, DNA-templated transcription by RNA polymerase II negative regulation of transcription elongation from RNA polymerase II promoter transcription, DNA-templated negative regulation of transcription, DNA-templated cell population proliferation stem cell differentiation negative regulation of stem cell differentiation positive regulation of viral transcription
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


25920


58202

Ensembl


ENSG00000188986


ENSMUSG00000013465

UniProt


Q8WX92


Q8C4Y3

RefSeq (mRNA)


NM_015456


NM_021393 NM_001310157

RefSeq (protein)


NP_056271


NP_001297086 NP_067368

Location (UCSC)

Chr 9: 137.26 – 137.27 Mb

Chr 2: 25.09 – 25.1 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Cofactor of BRCA1, also known as COBRA1, is a human gene^[5] that encodes NELF-B.

Function

[edit]

NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2; MIM 606026), either NELF-C or NELF-D (TH1L; MIM 605297), and NELF-E (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660).^[6] COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.^[7]

Interactions

[edit]

Cofactor of BRCA1 has been shown to interact with:

References

[edit]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000188986 – Ensembl, May 2017

^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000013465 – Ensembl, May 2017

^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

^ "Entrez Gene: COBRA1 cofactor of BRCA1".

^ Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (2003). "Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex". Molecular and Cellular Biology. 23 (6): 1863–1873. doi:10.1128/MCB.23.6.1863-1873.2003. PMC 149481. PMID 12612062.

^ Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R (2001). "BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations". The Journal of Cell Biology. 155 (6): 911–922. doi:10.1083/jcb.200108049. PMC 2150890. PMID 11739404.

^ Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R (Dec 2001). "BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations". J. Cell Biol. 155 (6): 911–921. doi:10.1083/jcb.200108049. PMC 2150890. PMID 11739404.

^ ^a ^b Zhong H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Q (Dec 2004). "COBRA1 inhibits AP-1 transcriptional activity in transfected cells". Biochem. Biophys. Res. Commun. 325 (2): 568–573. doi:10.1016/j.bbrc.2004.10.079. PMID 15530430.

^ Aiyar SE, Sun JL, Blair AL, Moskaluk CA, Lu YZ, Ye QN, Yamaguchi Y, Mukherjee A, Ren DM, Handa H, Li R (Sep 2004). "Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor". Genes Dev. 18 (17): 2134–2146. doi:10.1101/gad.1214104. PMC 515291. PMID 15342491.

^ ^a ^b Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (Mar 2003). "Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex". Mol. Cell. Biol. 23 (6): 1863–1873. doi:10.1128/MCB.23.6.1863-1873.2003. PMC 149481. PMID 12612062.

^ Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (Jan 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–167. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.

^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

Ping YH, Rana TM (2001). "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation". J. Biol. Chem. 276 (16): 12951–12958. doi:10.1074/jbc.M006130200. PMID 11112772.

Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–435. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.

Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–292. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.

Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R (2002). "BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations". J. Cell Biol. 155 (6): 911–921. doi:10.1083/jcb.200108049. PMC 2150890. PMID 11739404.

Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (2003). "Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex". Mol. Cell. Biol. 23 (6): 1863–1873. doi:10.1128/MCB.23.6.1863-1873.2003. PMC 149481. PMID 12612062.

Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–167. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.

Zhu J, Song S, Jiang Z, Yan J, Lu Q, Huang C, Ye Q (2005). "Characterization of COBRA1 in human breast cancer cell lines using a new polyclonal antibody against COBRA1". IUBMB Life. 56 (3): 161–166. doi:10.1080/15216540410001699826. PMID 15185750. S2CID 31137609.

Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–12135. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.

Aiyar SE, Sun JL, Blair AL, Moskaluk CA, Lu YZ, Ye QN, Yamaguchi Y, Mukherjee A, Ren DM, Handa H, Li R (2004). "Attenuation of estrogen receptor α-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor". Genes Dev. 18 (17): 2134–2146. doi:10.1101/gad.1214104. PMC 515291. PMID 15342491.

Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–2144. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.

Zhong H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Q (2005). "COBRA1 inhibits AP-1 transcriptional activity in transfected cells". Biochem. Biophys. Res. Commun. 325 (2): 568–573. doi:10.1016/j.bbrc.2004.10.079. PMID 15530430.

Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

McChesney PA, Aiyar SE, Lee OJ, Zaika A, Moskaluk C, Li R, El-Rifai W (2006). "Cofactor of BRCA1: a novel transcription factor regulator in upper gastrointestinal adenocarcinomas". Cancer Res. 66 (3): 1346–1353. doi:10.1158/0008-5472.CAN-05-3593. PMID 16452188.

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