Contents

EIF1

Protein-coding gene in the species Homo sapiens

EIF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2IF1, 4KZX, 4KZY
Identifiers
Aliases	EIF1, A121, EIF-1, ISO1, SUI1, eukaryotic translation initiation factor 1, Eukaryotic initiation factor 1
External IDs	MGI: 105125; HomoloGene: 130538; GeneCards: EIF1; OMA:EIF1 - orthologs
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q21.2 Start 41,688,885 bp[1] End 41,692,668 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11|11 D Start 100,210,711 bp[2] End 100,212,922 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm oocyte parotid gland parietal pleura tail of epididymis lower lobe of lung secondary oocyte vena cava caput epididymis visceral pleura Top expressed in granulocyte muscle of thigh morula blastocyst superior frontal gyrus right kidney ventricular zone neural tube extensor digitorum longus muscle embryo More reference expression data BioGPS n/a
Gene ontology Molecular function translation factor activity, RNA binding translation initiation factor activity RNA binding protein binding ribosomal small subunit binding Cellular component cytoplasm nucleus eukaryotic 43S preinitiation complex Biological process translational initiation response to stress dosage compensation by inactivation of X chromosome protein biosynthesis regulation of translational initiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10209 20918 Ensembl ENSG00000173812 ENSMUSG00000035530 UniProt P41567 P48024 RefSeq (mRNA) NM_005801 NM_011508 RefSeq (protein) NP_005792 NP_035638 Location (UCSC) Chr 17: 41.69 – 41.69 Mb Chr 11: 100.21 – 100.21 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1.^[5][6][7]

eIF1 interacts with the eukaryotic small (40S) ribosomal subunit and eIF3, and is a component of the 43S preinitiation complex (PIC).^[8] eIF1 and eIF1A bind cooperatively to the 40S to stabilize an "open" conformation of the preinitiation complex (PIC) during eukaryotic translation initiation.^[8] eIF1 binds to a region near the ribosomal P-site in the 40S subunit and functions in a manner similar to the structurally related bacterial counterpart IF3.^[9]

Structure[edit]

eIF1 is a conserved translation protein in all eukaryotic cells that is responsible for the investigation of codon-anticodon mismatches during the initiation of translation. In order to determine the structure of human eIF1, an experiment with N-terminal His tag and eIF1 are conducted via using NMR spectroscopy. Scientists have discovered a binding site by generating yeast mutation and study the neighbor conserved residues located in the same region. GST pull-down experiments has shown that eIF1 binds precisely to the p110 subunit of eIF3 as a result explaining eIF1 recruiting. ^[10]

Function[edit]

The function of eIf1 has some hidden aspects. However, in all eukaryotic cells initiation of mRNA translation starts with scanning via ribosomal 43S preinitiation complexes starting from the 5’ end of the mRNA. Next, induction via eIF1 and eIF1A are needed to disclose the conformation of the 40S subunit in order to induce DEAD-box RNA helicase eIF4A, its cofactor eIF4B, and eIF4G activity.^[11]   

See also[edit]

• Eukaryotic initiation factors^[12]

References[edit]

Further reading[edit]

External links[edit]

• EIF1 human gene location in the UCSC Genome Browser.
• EIF1 human gene details in the UCSC Genome Browser.
• Cap-dependent translation initiation from Nature Reviews Microbiology. A good image and overview of the function of initiation factors
• Overview of all the structural information available in the PDB for UniProt: P41567 (Eukaryotic translation initiation factor 1) at the PDBe-KB.

This protein-related article is a stub. You can help Wikipedia by expanding it.