| Cannabidiolic acid synthase | |||||||||
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| Identifiers | |||||||||
| EC no. | 1.21.3.8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Cannabidiolic acid synthase (EC 1.21.3.8, CBDA synthase) is an enzyme with systematic name cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming).[1][2] It is an oxidoreductase found in Cannabis sativa that catalyses the formation of cannabidiolate, a carboxylated precursorofcannabidiol.[2]
Cannabidiolic acid synthase consists of a single protein with a molecular mass of 74 kDa.[1] Its amino acid sequence is partly (40-50%) homologous to several other oxidoreductases,[2] such as berberine bridge enzymeinEschscholzia californica[3] and Nectarin VinNicotiana langsdorffii X N. sanderae.[4]
CBDA synthase has four binding sites; two for FAD and two for the substrate.[5]
Cannabidiolic acid synthase catalyses the production of cannabidiolate predominantly from cannabigerolate by stereospecific oxidative cyclization of the geranyl groupofcannabigerolic acid[2] according to the following chemical reaction:
Cannabinerolate can also be used as a substrate, but with lower efficiency (KM=0.137 mM) than cannabigerolate (KM=0.206 mM). It covalently binds FAD, and does require coenzymes & molecular oxygen for the oxidocyclization reaction.[1]
The optimum pH for CBDA synthase is 5.0.[2]
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Other oxidoreductases (EC 1.15–1.21)
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| 1.15: Acting on superoxide as acceptor |
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| 1.16: Oxidizing metal ions |
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| 1.17: Acting on CH or CH2 groups |
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| 1.18: Acting on iron–sulfur proteins as donors |
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| 1.19: Acting on reduced flavodoxin as donor |
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| 1.20: Acting on phosphorusorarsenic in donors |
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| 1.21: Acting on X-H and Y-H to form an X-Y bond |
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