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| Names | |
|---|---|
| Preferred IUPAC name
2,2′-(Piperazine-1,4-diyl)di(ethane-1-sulfonic acid) | |
| Other names
PIPES | |
| Identifiers | |
3D model (JSmol) |
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| ChemSpider |
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| ECHA InfoCard | 100.024.598 
|
PubChem CID |
|
| UNII | |
CompTox Dashboard (EPA) |
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| |
| |
| Properties | |
| C8H18N2O6S2 | |
| Molar mass | 302.37 |
| Appearance | White powder |
| Melting point | Decomposes above 300 °C |
| Boiling point | Decomposes |
| 1 g/L (100 °C) | |
| Hazards | |
| Occupational safety and health (OHS/OSH): | |
Main hazards |
Irritant |
| NFPA 704 (fire diamond) | |
| Safety data sheet (SDS) | External MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is a frequently used buffering agentinbiochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]
PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.[5]
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