| Diacylglycerol kinase | |||||||||
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DgkB, soluble DAGK from Staphylococcus aureus. α-helices in red, β-strands in yellow, coils in green.
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| Identifiers | |||||||||
| EC no. | 2.7.1.107 | ||||||||
| CAS no. | 60382-71-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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| Prokaryotic diacylglycerol kinase | |||||||||
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| Identifiers | |||||||||
| Symbol | DAGK_prokar | ||||||||
| Pfam | PF01219 | ||||||||
| InterPro | IPR000829 | ||||||||
| PROSITE | PDOC00820 | ||||||||
| OPM superfamily | 196 | ||||||||
| OPM protein | 4d2e | ||||||||
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| Diacylglycerol kinase catalytic domain | |||||||||
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| Identifiers | |||||||||
| Symbol | DAGK_cat | ||||||||
| Pfam | PF00781 | ||||||||
| Pfam clan | CL0240 | ||||||||
| InterPro | IPR001206 | ||||||||
| SMART | DAGKc | ||||||||
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| Diacylglycerol kinase accessory domain | |||||||||
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| Identifiers | |||||||||
| Symbol | DAGK_acc | ||||||||
| Pfam | PF00609 | ||||||||
| InterPro | IPR000756 | ||||||||
| SMART | DAGKa | ||||||||
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Diacylglycerol kinase (DGK or DAGK) is a family of enzymes that catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA), utilizing ATP as a source of the phosphate.[1] In non-stimulated cells, DGK activity is low, allowing DAG to be used for glycerophospholipid biosynthesis, but on receptor activation of the phosphoinositide pathway, DGK activity increases, driving the conversion of DAG to PA. As both lipids are thought to function as bioactive lipid signaling molecules with distinct cellular targets, DGK therefore occupies an important position, effectively serving as a switch by terminating the signalling of one lipid while simultaneously activating signalling by another.[2]
Inbacteria, DGK is very small (13 to 15 kDa) membrane protein which seems to contain three transmembrane domains.[3] The best conserved region is a stretch of 12 residues which are located in a cytoplasmic loop between the second and third transmembrane domains. Some Gram-positive bacteria also encode a soluble diacylglycerol kinase capable of reintroducing DAG into the phospholipid biosynthesis pathway. DAG accumulates in Gram-positive bacteria as a result of the transfer of glycerol-1-phosphate moieties from phosphatidylglyceroltolipotechoic acid.[4]
Currently, nine members of the DGK family have been cloned and identified. Although all family members have conserved catalytic domains and two cysteine rich domains, they are further classified into five groups according to the presence of additional functional domains and substrate specificity.[5] These are as follows:
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| Catabolism |
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| 2.7.1-2.7.4: phosphotransferase/kinase (PO4) |
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| 2.7.6: diphosphotransferase (P2O7) |
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| 2.7.7: nucleotidyltransferase (PO4-nucleoside) |
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| 2.7.8: miscellaneous |
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| 2.7.10-2.7.13: protein kinase (PO4; protein acceptor) |
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