Contents

Glutaryl-CoA dehydrogenase

Protein-coding gene in the species Homo sapiens

GCDH
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SIQ, 1SIR, 2R0M, 2R0N
Identifiers
Aliases	GCDH, ACAD5, GCD, glutaryl-CoA dehydrogenase, Glutaryl-Coenzyme A dehydrogenase
External IDs	OMIM: 608801; MGI: 104541; HomoloGene: 130; GeneCards: GCDH; OMA:GCDH - orthologs
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19p13.13 Start 12,891,160 bp[1] End 12,914,207 bp[1]
Gene location (Mouse) Chr. Chromosome 8 (mouse)[2] Band 8 C3|8 41.28 cM Start 85,613,022 bp[2] End 85,620,550 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right lobe of liver apex of heart left ventricle right ovary gastrocnemius muscle left ovary mucosa of transverse colon body of stomach muscle of thigh right adrenal gland Top expressed in right kidney human kidney left lobe of liver proximal tubule Ileal epithelium cardiac muscle tissue of left ventricle interventricular septum extraocular muscle choroid plexus of fourth ventricle extensor digitorum longus muscle More reference expression data BioGPS n/a
Gene ontology Molecular function oxidoreductase activity, acting on the CH-CH group of donors acyl-CoA dehydrogenase activity oxidoreductase activity glutaryl-CoA dehydrogenase activity fatty-acyl-CoA binding flavin adenine dinucleotide binding glutaryl-CoA hydrolase activity Cellular component mitochondrial matrix mitochondrion Biological process fatty-acyl-CoA biosynthetic process tryptophan metabolic process acyl-CoA metabolic process fatty acid oxidation lysine catabolic process fatty acid beta-oxidation using acyl-CoA dehydrogenase Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 2639 270076 Ensembl ENSG00000105607 ENSMUSG00000003809 UniProt Q92947 Q60759 RefSeq (mRNA) NM_000159 NM_013976 NM_001044744 NM_008097 RefSeq (protein) NP_000150 NP_039663 NP_001038209 NP_032123 Location (UCSC) Chr 19: 12.89 – 12.91 Mb Chr 8: 85.61 – 85.62 Mb PubMed search [3] [4]
Wikidata
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Glutaryl-CoA dehydrogenase (GCDH) is an enzyme encoded by the GCDH geneonchromosome 19. The protein belongs to the acyl-CoA dehydrogenase family (ACD). It catalyzes the oxidative decarboxylationofglutaryl-CoAtocrotonyl-CoA and carbon dioxide in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants.^[5]

Structure[edit]

GCDH is a tetramer with tetrahedral symmetry, which allows it to be seen as a dimer of dimers. Its structure is very similar to other ACDs but the overall polypeptide fold of the GCDH is made up of three domains: an alpha-helical bundle amino-terminal domain, a beta-sheet domain in the middle, and another alpha-helical domain at the carboxyl terminus. The flavin adenine dinucleotide (FAD) is located at the junction between the middle beta-strand and the carboxyl terminal alpha-helix domain of one subunit and the carboxyl-terminal domain of the neighboring subunit. The most distinct difference between GCDH and other ACDs in terms of structure is the carboxyl and amino-terminal regions of the monomer and in the loop between beta-strands 4 and 5 because it is only made up of four residues, whereas other ACDs have much more. The substrate-binding pocket is filled with a string of three water molecules, which gets displaced when the substrate binds to the enzyme. The binding pocket is also smaller than some of the other ACD binding pockets because it is responsible for the chain-length specificity of GCDH for alternate substrates.^[6] The GCDH gene is mapped onto 19p13.2 and has an exon count of 15.^[7]

Function[edit]

GCDH is mainly known for the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and carbon dioxide, which is common in the mitochondrial oxidation of lysine, tryptophan, and hydroxylysine. The way it completes this task is through a series of physical, chemical, and electron-transfer steps. It first binds glutaryl-CoA substrate to the oxidized form of the enzyme and abstracts the alpha-proton of the substrate by the Glu370 catalytic base. Hydride is then transferred from the beta-carbon of the substrate to the N(5) of the FAD, yielding the 2e⁻-reduced form of FAD. Thus, this allows for the decarboxylation of glutaconyl-CoA, an enzyme-bound intermediate, by breaking the Cγ-Cδ bond, resulting in formation of a dienolate anion, a proton, and CO₂. The dienolate intermediate is protonated, resulting in crotonyl-CoA and a release of products from the active site. Finally, the 2e⁻-reduced form of FAD is oxidized to two 1e⁻ steps by an external electron acceptor to complete the turnover.^[8]

Clinical significance[edit]

Mutations in the GCDH gene can lead to defects in the enzyme encoded by it which leads to the formation and accumulation of the metabolites glutaric acid and 3-hydroxyglutaric acid as well as glutarylcarnitine in body fluids, which essentially leads to glutaric aciduria type I, an autosomal recessive metabolic disorder. Symptoms for this disease include: macrocephaly, acute encephalitis-like crises, spasticity, dystonia, choreoathetosis, ataxia, dyskinesia and seizure and are prevalent one in every 100,000 individuals.^[7] Mutations in the carboxyl-terminal of GCDH have been most identified in patients with glutaric aciduria type I; more specifically, mutations in Ala389Val, Ala389Glu, Thr385Met, Ala377Val, and Ala377Thr all seem to be associated with the disorder because they dissociate to inactive monomers and/or dimers.^[6]

Interactions[edit]

GCDH has been seen to interact with:

• glutaryl-CoA

References[edit]

External links[edit]

• Glutaryl-CoA+dehydrogenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutaryl-CoA dehydrogenase, mitochondrial