Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues.[1]
Lys-N is becoming a popular protease used for protein digestion in proteomics experiments.[citation needed] The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions.[3] This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing.[3]
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